BamHI, HindIII, FseI, and PacI restriction sites are indicated by underscoring; a C vairiant is indicated in boldface type.
TABLE 2. Thermal cycle conditions for the three different kits used in real-time RT-PCR assays (Table view)
Program
No. of cycles
Temp (°C)
Incubation time (min:s)
Temp transition rate (°C/s)
Acquisition mode
HCV
1
1
50
25:00
20
None
2
1
94
2:00
20
None
3
5
93
3:00
20
None
55
15:00
2
None
72
15:00
20
None
4
42
93
3:00
20
None
60
45:00
20
Single
5
1
40
30:00
20
None
H5N1
1
1
42
30:00
20
None
2
1
92
3:00
20
None
3
5
92
10:00
20
None
45
30:00
20
None
72
1:00
20
None
4
40
92
10:00
20
None
60
30:00
20
Single
5
1
40
0:00
20
None
SARS-Cov2
1
1
42
30:00
20
None
2
1
92
3:00
20
None
3
5
92
10:00
20
None
52
20:00
2
None
72
30:00
20
None
40
92
5:00
20
None
4
60
30:00
20
Single
5
1
40
10:00
20
None
Acknowledgments
This study was supported in part by the SEPSDA project of the European Commission (under no. Sp22-CT-2004-003831), the National Natural Science Foundation of China (30371365 and 30571776), and the Capital Medicine Development Foundation of Beijing (2002-3041).
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